Launch from Cape Canaveral (KSC), Launch Complex 39-A at NASA's Kennedy Space Center in Florida. The Crew Dragon Endeavour docked 21 hours later with the International Space Station. The spacecraft splashed down east of Jacksonville in the Atlantic.

It was the first all-private astronaut mission to the International Space Station (ISS) and a pivotal step toward Axiom Station, the world's first commercial space station. The Axiom Space mission to the International Space Station focused on science, education, and STEM outreach, conducting more than 25 experiments, collecting critical data, and dedicating more than 100 hours of human tended research during their 17-day mission.

Among the science experiments were:

TESSERAE (Tessellated Electromagnetic Space Structures for the Exploration of Reconfigurable, Adaptive Environments) is a multi-year research program exploring self-assembly methods for in-space construction.

Using a human cancer stem cell nano-bioreactor model (a vessel that accelerates cell growth conditions) and cancer stem cell reporter system, this investigation will leverage the accelerated aging aspects of the microgravity environment to evaluate early pre-cancer and cancer changes in tumor organoids. This cellular biology project is focused on identifying biomarkers for early detection and supports future aims of cancer stem cell research on the ISS. The Ax-1 crew will study cell samples under a high-resolution microscope to determine cell cycle activity in cancer growth.

The Japan Manned Space Systems Corporation (JAMSS) photocatalyst air purification device was launched on Ax-1 for a technical demonstration to verify the performance of the improved filter. This experiment also deployed a control device without photocatalytic LEDs, to evaluate the JAMSS photocatalytic filter. With an improved air filter, the JAMSS device was able to clean the air on the ISS and remove cabin odor, using the power of light to safely convert volatile compounds in the air into carbon dioxide and water with the goal of improving the quality of life for living and working in space. JAMSS was be the only Japanese company with research on the Ax-1 mission.

Translational Research Institute for Space Health (TRISH) is conducting biomedical research on each of the crewmembers both before the launch and after their return to Earth. A variety of data will be collected, including physiological data, a battery of cognitive tests, balance and perception tests, and visual acuity tests. Increasing human health and performance is a goal of TRISH's EXPAND (Enhancing eXploration Platforms and ANalog Definition) program, which compiles health data from private spaceflight into a centralized research database and broadens understanding of how space impacts human health and how to prepare humans for the demands of long-distance and long-duration space travel, including to the Moon and eventually, to Mars.