Launch from Cape Canaveral (KSC), Launch Complex 39-A at NASA's Kennedy Space Center in Florida. The travel group landed 50 kilometers northeast of Kennedy Space Center.

Inspiration4 marked the world's first all-civilian mission to orbit. The crew conducted experiments designed to expand our knowledge of the universe. Crew Dragon's cargo capacity was allocated for both crew essentials as well as scientific equipment dedicated to micro-gravity research and experimentation.

Inspiration4 assigned the maximum possible mass towards this valuable research, providing access to space for inspiring projects that are otherwise unable to overcome the high barriers of traditional space-based research. The mission was planned to include ultrasounds, microbe samples and a variety of in-flight health experiments (measure fluid shifts, record ECG activity, blood oxygen levels, heart rates, etc.) on the human bodies of ordinary citizens who have not been previously carefully screened and exhaustively trained as professional astronauts. The study of the effects of spaceflight on human health and performance was done in collaboration with SpaceX, the Translational Research Institute for Space Health (TRISH) at Baylor College of Medicine and investigators at Weill Cornell Medicine. During the journey, an alarm had sounded, and was found to be associated with an apparent toilet malfunction.